Inducible Fhit expression in H1299 cells
Variable analysis
- Inducible expression of Fhit cDNA and protein (D1) or an empty vector (E1)
- Not explicitly mentioned
- H1299 lung carcinoma cells maintained in DMEM medium with 10% FBS, zeocin, gentamicin and geneticin
- Fhit expression induced by addition of ponasterone A (2 μM) to the growth medium for 48 h
- Positive control: H1299 cells inducibly expressing Fhit cDNA and protein (D1)
- Negative control: H1299 cells inducibly expressing an empty vector (E1)
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