The engineered H1299 lung carcinoma cells, which inducibly express Fhit cDNA and protein (D1) or an empty vector (E1) have been described [20 (link)]. H1299 cells were maintained in DMEM medium with 10% FBS, zeocin, gentamicin and geneticin. Fhit expression was induced by addition of ponasterone A (2 μM) (Life Technologies) to the growth medium for 48 h.
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