Antibody Generation and Validation

NgBR rabbit monoclonal antibody (CloneID: EPR8668) was generated by Epitomics (Burlingame, CA, USA) as a collaboration project. NgBR rabbit polyclonal antibody was generated as described previously [19 (link)] and was used for immunohistochemistry analysis. Rabbit polyclonal antibodies for phospho-AKT (Ser473), total Akt, Phospho-MDM2 (Ser166), cyclinD1, p21, phos-Rb (Ser807/811), HA-Tag rabbit monoclonal antibody, Histone H3, and β-actin and all the secondary antibodies were purchased from Cell Signaling (Danvers, MA, USA). Rabbit polyclonal antibodies for p53, ubiquitin, CDK6, and β-tublin were purchased from ProteinTech (Chicago, IL, USA). Rabbit polyclonal antibody for total MDM2 was purchased from Anbo Biotechnology (San Francisco, CA, USA).

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Dong C., Zhao B., Long F., Liu Y., Liu Z., Li S., Yang X., Sun D., Wang H., Liu Q., Liang R., Li Y., Gao Z., Shao S., Miao Q.R, & Wang L. (2016). Nogo-B receptor promotes the chemoresistance of human hepatocellular carcinoma via the ubiquitination of p53 protein. Oncotarget, 7(8), 8850-8865.

Publication 2016

Phospho-MDM2 (Ser166 HA-Tag rabbit monoclonal antibody Histone H3 β-actin

Actin Antibodies Antibody Cdk6 Histone h3 Immunohistochemistry Mdm2 Monoclonal antibody Rabbit Ubiquitin

Corresponding Organization : National Center for Nanoscience and Technology

Other organizations : Second Affiliated Hospital of Dalian Medical University, Dalian Medical University, Dalian Institute of Chemical Physics, Affiliated Zhongshan Hospital of Dalian University

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Variable analysis

independent variables

NgBR rabbit monoclonal antibody (CloneID: EPR8668) generated by Epitomics
NgBR rabbit polyclonal antibody generated as described previously [19]

dependent variables

Immunohistochemistry analysis

control variables

Rabbit polyclonal antibodies for phospho-AKT (Ser473), total Akt, Phospho-MDM2 (Ser166), cyclinD1, p21, phos-Rb (Ser807/811), HA-Tag rabbit monoclonal antibody, Histone H3, and β-actin and all the secondary antibodies purchased from Cell Signaling
Rabbit polyclonal antibodies for p53, ubiquitin, CDK6, and β-tublin purchased from ProteinTech
Rabbit polyclonal antibody for total MDM2 purchased from Anbo Biotechnology

positive controls

Not specified

negative controls

Not specified

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