Drosophila Husbandry and Genetics

Flies were raised on standard agar, cornmeal, molasses, and yeast food containing 1.5% Nipagin bought from IMBA (Vienna, Austria). Adults were placed in cages in a Percival DR 36VL incubator maintained at 29° and 65% humidity, and embryos were collected on standard plates prepared in house from apple juice, sugar, agar, and Nipagin, and treated with yeast from Lesaffre (Marcq, France). This applies to all experiments except the QF2 movie, whose fly husbandry conditions are described below. repo-GAL4 and QUAS-CD8::GFP were obtained from the Bloomington Drosophila Stock Center, UAS-moe::mCherry from P. Martin (Millard and Martin 2008 (link)), hml-dsRed from K. Brückner, and srp-GAL4 UAS-2xeGFP from R. Reuter.