Inhibiting STAT3 in Colorectal Cancer

The human colorectal cancer cell lines HCT116 and SW480 (Obtained from Zhong Qiao Xin Zhou Biotechnology Co., Ltd., China) were cultured in DMEM (Gibco BRL, Grand Island, NY) complete medium containing 10%, FBS and 1% penicillin/streptomycin (Zhong Qiao Xin Zhou Biotechnology Co., Ltd., China) and incubated in an incubator containing 37 °C and 5% CO2. Fresh medium was exchanged every 2 days, and when the cell density reached 80–90%, the cells were digested with trypsin solution for subculture or plate laying. HO-3867 (10 µmol/l, MedChemExpress LLC, China) is a STAT3 inhibitor used to inhibit the expression of STAT3 [32 (link)].

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Yu J., Yang H., Zhang L., Ran S., Shi Q., Peng P., Liu Q, & Song L. (2024). Effect and potential mechanism of oncometabolite succinate promotes distant metastasis of colorectal cancer by activating STAT3. BMC Gastroenterology, 24, 106.

Publication 2024

HCT116 SW480 DMEM penicillin/streptomycin HO-3867

Corresponding Organization :

Other organizations : Guiyang Medical University, Affiliated Hospital of Guizhou Medical University

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Variable analysis

independent variables

HO-3867 (10 µmol/l, MedChemExpress LLC, China)

dependent variables

Expression of STAT3

control variables

HCT116 and SW480 human colorectal cancer cell lines
DMEM (Gibco BRL, Grand Island, NY) complete medium containing 10% FBS and 1% penicillin/streptomycin
Incubator containing 37°C and 5% CO2
Fresh medium exchanged every 2 days
Cell density reaching 80-90% before subculture or plate laying

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