RNA Extraction from FFPE Tissues

We followed previously published protocol for the extraction of RNA from FFPE tissues (11 (link)). Based on our previous experience, we used 4 × 10 μm-thick sections to extract total RNA from FFPE samples. For rapid purification, PureLink FFPE Total RNA Isolation Kit was used (Invitrogen, Thermo Fischer Scientific, Foster City, CA) according to the manufacturer's recommendations. The RNA data quality was assessed by 260/280 absorption signal ratio and the RIN number.

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Sigdel T., Nguyen M., Liberto J., Dobi D., Junger H., Vincenti F., Laszik Z, & Sarwal M.M. (2019). Assessment of 19 Genes and Validation of CRM Gene Panel for Quantitative Transcriptional Analysis of Molecular Rejection and Inflammation in Archival Kidney Transplant Biopsies. Frontiers in Medicine, 6, 213.

Publication 2019

PureLink FFPE Total RNA Isolation Kit

Isolation Tissues

Corresponding Organization : University of California, San Francisco

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Variable analysis

independent variables

The use of 4 × 10 μm-thick sections for RNA extraction

dependent variables

Total RNA extracted from FFPE samples
260/280 absorption signal ratio
RIN number

control variables

Previously published protocol for the extraction of RNA from FFPE tissues (11)
PureLink FFPE Total RNA Isolation Kit used according to the manufacturer's recommendations

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