Flow Cytometry Analysis of MSC Antigens

Antigen expression was performed as previously described [14 (link)] and assessed with FACSCanto II instrument (Becton Dickinson, Franklin Lakes, NJ, USA) and by FACSDiva software 6.0 (Becton Dickinson). Briefly, at passage 1, 0.5–1 × 10⁵ of MSCs for each antigen were washed with PBS, centrifuged, and incubated in flow cytometry buffer, adding fluorescein isothiocyanate (FITC)-conjugated antibody against CD-31, 45, 34, 44, 73, 90, and 105. FITC-conjugated nonspecific immunoglobulin G (IgG) was used as control (Bio-Legend, San Diego, CA, USA).

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Salamanna F., Contartese D., Borsari V., Pagani S., Sartori M., Tschon M., Griffoni C., Giavaresi G., Tedesco G., Barbanti Brodano G., Gasbarrini A, & Fini M. (2023). Gender-Specific Differences in Human Vertebral Bone Marrow Clot. International Journal of Molecular Sciences, 24(14), 11856.

Publication 2023

FACSCanto II instrument FITC-conjugated nonspecific immunoglobulin G (IgG)

Antibody Antigen Buffer Flow cytometry Fluorescein Immunoglobulin g Isothiocyanate

Corresponding Organization : Istituto Ortopedico Rizzoli

Other organizations : Istituti di Ricovero e Cura a Carattere Scientifico

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Variable analysis

independent variables

Passage 1 of MSCs

dependent variables

Antigen expression of CD-31, 45, 34, 44, 73, 90, and 105

control variables

FITC-conjugated nonspecific immunoglobulin G (IgG)

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