To the solution of peptide acid (1.0 equiv.) obtained from resins 7, 8 or 19 and HATU (4.0 equiv.) dissolved in DMF/CH2Cl2 (1 : 1, peptide concentration 0.02 M), propargylamine (3.0 equiv.) and Et3N (5.0 equiv.) were added. After stirring for 1 h, CH2Cl2 was removed from the reaction mixture under reduced pressure. The remaining mixture was purified by HPLC with a Vydac C18 column.
Protocol detail
Synthesis of Propargylamine Peptide Conjugates
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Corresponding Organization :
Other organizations : National Tsing Hua University, National Synchrotron Radiation Research Center, National Taiwan University, Vilnius University
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Variable analysis
independent variables
- Peptide acid (1.0 equiv.) obtained from resins 7, 8 or 19
- HATU (4.0 equiv.)
- Propargylamine (3.0 equiv.)
- Et3N (5.0 equiv.)
- Purified peptide product
- DMF/CH2Cl2 (1 : 1) as the reaction solvent
- Peptide concentration in the reaction mixture (0.02 M)
- No positive or negative controls were explicitly mentioned.
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