Human pre–B cell leukemic line NALM-6 cells (ATCC) were cultured in T cell medium (RPMI plus 10% FBS) and used as CD19+ target cells. The HBL-1 DLBCL cell line expressing CD58 was prepared as described previously (35 (link)). Parental HBL-1 and HBL-1–CD58+ were cultured in IMDM plus 10% FBS.
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