Alcohol, Saccharin, and Cocaine Exposure

Ethanol (Sigma-Aldrich, Saint Louis, MO, 95%, density = 0.816) for intraperitoneally (i.p.) injections was prepared in sterile saline at different concentrations (10 ml/kg). Sterile saline was injected i.p. at 10 ml/kg. Alcohol was prepared at 3%, 6%, and 9% (v/v%) in acidified drinking water. Saccharin (Sigma-Aldrich, Saint Louis, MO) was prepared at 0.06% and 0.12% (w/v%) in acidified drinking water. Cocaine HCl (Sigma-Aldrich, Saint Louis, MO) was prepared using sterile saline and injected i.p. (10 ml/kg) at different concentrations. Antibodies and mouse cell lines utilized in the present study were described previously [26 (link), 27 (link)].