Quantification of Atropine in Plasma

Atropine concentration in plasma was quantified at the National Veterinary Institute (SVA) in Uppsala, Sweden, using ultra–high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). The system was composed of an Acquity UHPLC coupled to a TQS Micro tandem quadrupole mass spectrometer with an electrospray interface operating in the positive mode (Waters Corporation, Milford, MA, United States). The calibration range was 0.05–60 μg/L plasma. The precision (relative standard deviation) was in the range of 2.1–8.3% and the recovery was 95.5–98%. The analytical method is thoroughly described in Ström et al.'s work (5 (link)).