The procedure was conducted as previously described38 (link)–40 (link). Briefly, age-, sex- and weight-matched animals were used and housed together for at least 14 days prior to surgery. Each mouse’s corresponding lateral aspects were shaved, incisions were made from the fore-limb joint to the hind-limb joint and the subcutaneous fascia was bluntly dissected to create 0.5 cm of free skin. Fore- and hind-limb joints were joined and the dorsal and ventral skins were approximated by continuous suture using mononylon 5.0 (Ethicon).
Protocol detail
Surgical Skin Flap in Mice
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Top 5 similar protocols
Variable analysis
independent variables
- Weight
- Skin flap healing
- Housing duration (at least 14 days prior to surgery)
- Shaving of lateral aspects
- Incision length (from fore-limb joint to hind-limb joint)
- Subcutaneous fascia dissection (0.5 cm of free skin)
- Fore- and hind-limb joint joining
- Suture material (mononylon 5.0)
- Suture technique (continuous)
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!