Integrin Antibody Characterization

The antibodies used were the mouse anti-human integrin β₁ antibodies TS2/16 (activating; a gift from Francisco Sanchez-Madrid, Universidad Autonoma de Madrid, Spain), 12G10 (activating) (Mould et al., 1995b (link)), and K20 (nonfunction-modulating, Immunotech); rat anti-human integrin β₁ antibodies mAb13 (inhibitory) (Akiyama et al., 1989 (link)) and 9EG7 (activating, Pharmingen); rat anti-human integrin α₅ mAb16 (inhibitory) (Akiyama et al., 1989 (link)) and mAb11 (nonfunction-modulating) (Miyamoto et al., 1995 (link)); mouse anti-human integrin α₅ antibodies SNAKA52 (inhibitory) and JBS5 (inhibitory, Serotec); mouse anti-human integrin α_V L230 (inhibitory, ATCC); and polyclonal rabbit anti-human fibronectin Rb745 (Cukierman et al., 2001 (link)).

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Clark K., Pankov R., Travis M.A., Askari J.A., Mould A.P., Craig S.E., Newham P., Yamada K.M, & Humphries M.J. (2004). A Specific α5β1 Integrin Conformation Promotes Directional Integrin Translocation and Fibronectin Matrix Formation. Journal of cell science, 118(Pt 2), 291-300.

Publication 2004

Anti antibodies Antibodies Fibronectin Human Inhibitory Integrin Integrin αv Mould Mouse Rabbit

Corresponding Organization :

Other organizations : National Institutes of Health, University of Manchester, Wellcome Centre for Cell-Matrix Research

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Protocol cited in 8 other protocols

Variable analysis

independent variables

Mouse anti-human integrin β1 antibodies TS2/16 (activating)
Mouse anti-human integrin β1 antibodies 12G10 (activating)
Mouse anti-human integrin β1 antibodies K20 (nonfunction-modulating)
Rat anti-human integrin β1 antibodies mAb13 (inhibitory)
Rat anti-human integrin β1 antibodies 9EG7 (activating)
Rat anti-human integrin α5 mAb16 (inhibitory)
Rat anti-human integrin α5 mAb11 (nonfunction-modulating)
Mouse anti-human integrin α5 antibodies SNAKA52 (inhibitory)
Mouse anti-human integrin α5 antibodies JBS5 (inhibitory)
Mouse anti-human integrin αV L230 (inhibitory)
Polyclonal rabbit anti-human fibronectin Rb745

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: not specified
Negative controls: not specified

Annotations

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