The viability of primary cortical neurons was estimated by Hoechst 33258 and propidium iodide (Hoechst/PI; Sigma-Adrich) co-staining and visualized using a fluorescence microscope Olympus IX71 as previously described (Rozzi et al. 2014 (link)). Hoechst/PI-positive cells were then counted using ImageJ and expressed as a percentage of the total number of neurons.
Gp120 toxicity was evaluated by measuring lactate dehydrogenase (LDH) release using CytoTox 96® NonRadioactive Cytotoxicity Assay, according to manufacturer’s instructions (Promega Corp, Madison, WI). LDH quantitation was done by measuring wavelength absorbance at 490 nm. Data are normalized to the amount of LDH released from vehicle-treated cells.
Gp120 toxicity was evaluated by measuring lactate dehydrogenase (LDH) release using CytoTox 96® NonRadioactive Cytotoxicity Assay, according to manufacturer’s instructions (Promega Corp, Madison, WI). LDH quantitation was done by measuring wavelength absorbance at 490 nm. Data are normalized to the amount of LDH released from vehicle-treated cells.
