Statistical analyses were performed using Graph Pad Prism V8.0.1. (GraphPad Software, Inc., San Diego, CA, USA). The normality of the data was checked using the Shapiro–Wilk test and the appropriate statistical assay was accordingly applied. For parametric data an unpaired t test with Welch’s correction was used, whereas, for non-parametric data, a Mann–Whitney test was applied. Statistical significance was set at p < 0.05 for all the tests.
Regarding the IR data, the differences between groups were statistically analyzed with the software package Graph Pad Prism V8.0.1. (GraphPad Software, San Diego, CA, USA). All data were presented as mean ± SD. Statistical significance among groups was evaluated using Student’s t-test. Statistical significance was set at p < 0.05. Moreover, a PCA was performed as an unsupervised multivariate approach to compare the spectral profiles of CTRL and PRE experimental groups (OriginPro 2018b software, OriginLab Corporation, Northampton, MA, USA) [29 (link),31 (link)].
Regarding the IR data, the differences between groups were statistically analyzed with the software package Graph Pad Prism V8.0.1. (GraphPad Software, San Diego, CA, USA). All data were presented as mean ± SD. Statistical significance among groups was evaluated using Student’s t-test. Statistical significance was set at p < 0.05. Moreover, a PCA was performed as an unsupervised multivariate approach to compare the spectral profiles of CTRL and PRE experimental groups (OriginPro 2018b software, OriginLab Corporation, Northampton, MA, USA) [29 (link),31 (link)].
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