Cell proliferation was analyzed using RTCA [19 (link)]. First, 50 μl of cell culture medium was added to E-Plate 16 dishes (ACEA Biosciences Inc, CA, USA), and the background impedance was measured and displayed as the cell index. Then, cells were seeded in these dishes at a density of 5,000 cells per well, and 200 μl of complete medium was added. After 24 h, cells were treated with 60 μM Res. The cell index, representing the change in impedance and the proliferation of cells, was then obtained.
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