One microgram of DNA was treated with bisulfite using the EpiTect Bisulfite Kit (Qiagen, Hilden, Germany). DNA methylation of CpG sites in the promoter region of the OXT gene (chr20: 3,052,266–3,053,162; hg19 build) was analyzed using EpiTYPER (MassARRAY system; Agena Biosciences., San Diego, CA, USA) according to the manufacturer’s instructions. Forward (aggaagagagTTTTTTTGTTTTATTTTAGTGGTTTAGG) and reverse (cagtaatacgactcactatagggagaaggctTCTTACCTCCCAAAAAACAATTCTA) primers corresponding to chr20:3,052,009–3,052,392 were designed using EpiDesigner (Agena Bioscience, San Diego, CA, USA), and the spectrum characteristics were validated with RSeqMeth [28 (link)]. Further details were described in a previous study [22 (link)] where SN and AKS contributed as co-authors.
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