DNA Methylation Analysis of OXT Gene

One microgram of DNA was treated with bisulfite using the EpiTect Bisulfite Kit (Qiagen, Hilden, Germany). DNA methylation of CpG sites in the promoter region of the OXT gene (chr20: 3,052,266–3,053,162; hg19 build) was analyzed using EpiTYPER (MassARRAY system; Agena Biosciences., San Diego, CA, USA) according to the manufacturer’s instructions. Forward (aggaagagagTTTTTTTGTTTTATTTTAGTGGTTTAGG) and reverse (cagtaatacgactcactatagggagaaggctTCTTACCTCCCAAAAAACAATTCTA) primers corresponding to chr20:3,052,009–3,052,392 were designed using EpiDesigner (Agena Bioscience, San Diego, CA, USA), and the spectrum characteristics were validated with RSeqMeth [28 (link)]. Further details were described in a previous study [22 (link)] where SN and AKS contributed as co-authors.

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Nishitani S., Fujisawa T.X., Hiraoka D., Makita K., Takiguchi S., Hamamura S., Yao A., Shimada K., Smith A.K, & Tomoda A. (2021). A multi-modal MRI analysis of brain structure and function in relation to OXT methylation in maltreated children and adolescents. Translational Psychiatry, 11, 589.

Publication 2021

EpiTect Bisulfite Kit EpiTYPER EpiDesigner

Bisulfite Dna methylation Gene Primers

Corresponding Organization :

Other organizations : University of Fukui, University of Fukui Hospital, Kanazawa University, Chiba University, Hamamatsu University School of Medicine, Osaka University, Emory University

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Variable analysis

independent variables

Bisulfite treatment using the EpiTect Bisulfite Kit (Qiagen, Hilden, Germany)

dependent variables

DNA methylation of CpG sites in the promoter region of the OXT gene (chr20: 3,052,266–3,053,162; hg19 build)

control variables

Primers corresponding to chr20:3,052,009–3,052,392 were designed using EpiDesigner (Agena Bioscience, San Diego, CA, USA), and the spectrum characteristics were validated with RSeqMeth

controls

Positive control: None mentioned
Negative control: None mentioned

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