Magnetic Tweezers for DNA Manipulation

The custom made MT setup was previously described (27–30 (link)), and consisted of an inverted optical microscope equipped with an oil-immersion objective (NIKON 100x, NA = 1.25) mounted on a piezoelectric focusing system (PIFoc, Physik Instrumente, Bresso, Italy). The objective, coupled with a 15 cm focal-length lens, led to a 75x magnification. The magnetic field was generated by two permanent neodymium magnets placed above the flow chamber and two piezoelectric motors controlled the position of the magnets along the optical axis (z-direction) and the rotation around the same axis in order to apply a stretching force, or a torque, to the torsionally constrained DNA.

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Salerno D., Marrano C.A., Cassina V., Cristofalo M., Shao Q., Finzi L., Mantegazza F, & Dunlap D. (2021). Nanomechanics of negatively supercoiled diaminopurine-substituted DNA. Nucleic Acids Research, 49(20), 11778-11786.

Publication 2021

PIFoc

Along Axis Immersion Lens Magnetic field Neodymium Optical microscope Torque

Corresponding Organization : Emory University

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Variable analysis

independent variables

Position of magnets along the optical axis (z-direction)
Rotation of magnets around the optical axis

dependent variables

Stretching force applied to the torsionally constrained DNA
Torque applied to the torsionally constrained DNA

control variables

Inverted optical microscope equipped with an oil-immersion objective (NIKON 100x, NA = 1.25) mounted on a piezoelectric focusing system (PIFoc, Physik Instrumente, Bresso, Italy)
Objective coupled with a 15 cm focal-length lens, leading to a 75x magnification
Permanent neodymium magnets placed above the flow chamber

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