(-)-Nicotine tartrate (MP Biomedicals, Solon, OH.) was dissolved in 0.9% saline. Nicotine was administered subcutaneously via osmotic minipumps (Alzet model 2002, Cupertino, CA) at a dose of 18 mg/kg/d for 14 days. This dose, reported as freebase weight and based off of previous work [16 (link), 21 (link)–23 (link)], corresponds to plasma levels of ~0.3 μM [24 (link)], a concentration similar to that observed in human smokers consuming an average of 17 cigarettes a day (plasma levels between 0.06 and 0.31 μM) [24 (link)].
Protocol detail
Chronic Nicotine Administration via Osmotic Pumps
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Other organizations : University of Kentucky, University of South Carolina
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Variable analysis
independent variables
- Nicotine dose (18 mg/kg/d)
- Plasma nicotine levels
- Nicotine administration method (subcutaneous via osmotic minipumps)
- Duration of nicotine administration (14 days)
- Positive control: Plasma nicotine levels observed in human smokers consuming an average of 17 cigarettes per day (0.06 to 0.31 μM)
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