Murine Mammary Tumor Cell Lines

Animal care and use were in accordance with institutional guidelines. Female, FVB/N-TgN (MMTVneu)-202Mul mice (TgMMTV-neu) (7 weeks old; median weight: 18 g, range: 15.4-18.5 g; Jackson Laboratory) or FVB-Tg(C3-1-TAg)cJeg/Jeg (C3(1)-Tag) mice (6 weeks old; median weight: 18.5 g, range: 16.5-18.5 g; provided by Dr. Jeff Green, NCI) were used in this study. Tumors derived from the C3(1)Tag mouse exhibit a basal phenotype and those derived from the TgMMTV-neu mouse exhibit a luminal/neu phenotype (16 (link)). The mouse mammary tumor cell lines MMC and M6 were derived from spontaneous mammary tumors from TgMMTV-neu (17 (link)) and C3(1)-Tag (18 (link)) mice, respectively. Both cells lines were authenticated before use. The MMC cell line was verified to express rat neu by flow cytometry and the M6 cell line was verified to express the SV40 antigen by Western blot and the estrogen receptor by RT-PCR.

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Cecil D.L., Slota M., O’Meara M.M., Curtis B.C., Gad E., Dang Y., Herendeen D., Rastetter L, & Disis M.L. (2016). Immunization against HIF-1α inhibits the growth of basal mammary tumors and targets mammary stem cells in vivo. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(13), 3396-3404.

Publication 2016

FVB/N-TgN (MMTVneu)-202Mul mice (TgMMTV-neu)

Animal Antigen Cell line Estrogen receptor Female Flow cytometry Mammary Mammary tumors Mice Phenotype Rt pcr Sv40 Tumor cell lines Tumors Western blot

Corresponding Organization : University of Washington

Other organizations : Seagen (United States)

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Variable analysis

independent variables

Mouse strain: FVB/N-TgN (MMTVneu)-202Mul mice (TgMMTV-neu) or FVB-Tg(C3-1-TAg)cJeg/Jeg (C3(1)-Tag) mice

dependent variables

Tumor phenotype: basal for C3(1)Tag mice and luminal/neu for TgMMTV-neu mice

control variables

Age of mice: 7 weeks for TgMMTV-neu and 6 weeks for C3(1)-Tag
Weight of mice: median weight of 18 g for both strains

controls

Positive control: The mouse mammary tumor cell lines MMC and M6 were derived from spontaneous mammary tumors from TgMMTV-neu and C3(1)-Tag mice, respectively
Verification: The MMC cell line was verified to express rat neu by flow cytometry, and the M6 cell line was verified to express the SV40 antigen by Western blot and the estrogen receptor by RT-PCR

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