Multilocus Sequence Typing of Bartonella bovis

Strains identified as B. bovis based on gltA sequences were further analyzed using MLST for eight additional loci (16S rRNA, ftsZ, groEL, nuoG, ribC, rpoB, ssrA, and ITS). Primers and other related information are provided in Table 2. We randomly selected one to three strains from each region of a country where we found bartonella to be present in the animals. Including the two reference strains from France and USA, a total of 28 strains were characterized for the MLST analysis (Table 3). A neighbor-joining tree based on the concatenated MLST alleles was constructed using the Clustal W program within MegAlign of the Lasergene package.
Newly-identified alleles from the current study were submitted to GenBank with the following accession numbers: KF193407 - KF193413, KF199895 - KF199899, KF212449 - KF212460, KF218206 - KF218209, KF218210 - KF218216, KF218217 - KF218224, KF218225 - KF218229, and KF218230 - KF218236 for ftsZ, gltA, groEL, nuoG, ribC, rpoB, ssrA, and ITS, respectively.

Free full text: Click here

Bai Y., Malania L., Alvarez Castillo D., Moran D., Boonmar S., Chanlun A., Suksawat F., Maruyama S., Knobel D, & Kosoy M. (2013). Global Distribution of Bartonella Infections in Domestic Bovine and Characterization of Bartonella bovis Strains Using Multi-Locus Sequence Typing. PLoS ONE, 8(11), e80894.

Publication 2013

16s rrna Alleles Animals Bartonella Based sequences Primers Ssra Strains Tree

Corresponding Organization :

Other organizations : Centers for Disease Control and Prevention, Universidad del Valle de Guatemala, Ministry of Public Health, Khon Kaen University, Nihon University, Centers for Disease Control and Prevention, Kenya Medical Research Institute, University of Pretoria

Top 5 similar protocols

Protocol cited in 6 other protocols

Variable analysis

independent variables

Strains identified as B. bovis based on gltA sequences

dependent variables

MLST analysis for eight additional loci (16S rRNA, ftsZ, groEL, nuoG, ribC, rpoB, ssrA, and ITS)

control variables

Two reference strains from France and USA

controls

Positive control: Two reference strains from France and USA
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!