Inflammatory State Quantification in Murine Model

The inflammatory state of the mice exposed to Pa-LPS and/or SAHA was quantified by measuring BALF or serum cytokine levels (IL-6) and neutrophil (MPO, myeloperoxidase) activity by sandwich ELISAs (R&D and Hycult Biotechnology respectively), as recently described [10 (link), 16 (link)]. The flow cytometry analysis of BALF samples from each murine experimental group was performed as previously described [10 (link), 16 (link), 21 (link)] and was used to quantify the percentage changes in number of CD4 + FoxP3+ T cells in BALF-cells. The BD FACS Caliber instrument and BD Cell Quest Pro software was used for acquisition and analysis of the data.

Free full text: Click here

Bodas M., Mazur S., Min T, & Vij N. (2018). Inhibition of histone-deacetylase activity rescues inflammatory cystic fibrosis lung disease by modulating innate and adaptive immune responses. Respiratory Research, 19, 2.

Publication 2018

sandwich ELISAs BD FACS Caliber instrument BD Cell Quest Pro

Cd4 cells number Cell Cytokine Elisas Flow cytometry Inflammatory Murine Myeloperoxidase Neutrophil Saha Serum

Corresponding Organization :

Other organizations : Central Michigan University, Johns Hopkins Medicine, Johns Hopkins University

Top 5 similar protocols

Variable analysis

independent variables

Exposure to Pa-LPS
Exposure to SAHA

dependent variables

BALF or serum cytokine levels (IL-6)
Neutrophil (MPO, myeloperoxidase) activity
Percentage changes in number of CD4 + FoxP3+ T cells in BALF-cells

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the input text.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!