Images in Figures 1F, G , 3C, G , 6J , Figure 5E ; Figure 5—figure supplement 4A, B and Figure 6—figure supplement 1E were acquired with a Leica M205FA stereo microscope and display live fluorescence of fluorescent proteins. High-resolution optical sections were obtained with a Leica SP8 confocal microscope or a Zeiss AxioObserver 7 equipped with an Apotome and processed using Fiji (Schindelin et al., 2012 (link)). Images in Figures 1B,C and D ,2A, D and 4A , 6B,E,G ; and Figure 1—figure supplement 1A, B ; Figure 2—figure supplement Figure 2—figure supplements 1A and 2A,B , Figure 5—figure supplement 1A ; Figure 6—figure supplement 1B,D and Video 1 are z-projections. Figure 5A and Figure 5—figure supplement 2D show single z-planes. Video 2 was recorded with a Leica M205FA stereo microscope. Movie annotations were added using the Annotate_movie plugin (Daetwyler et al., 2020 (link)).
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