Each mouse was placed in a square white acrylic arena (50 × 50 cm), facing a wall, for 20 min and allowed to freely explore the arena. The area was lighted with white light (100 lx) (33 (link)). Video recordings were taken and analyzed using Biobserve Viewer III (Biobserve GmbH, Germany). A virtual square of equal distance from the periphery (25 × 25 cm) was defined as the “central zone,” and the outer part of the arena was defined as “peripheral zone.” Distance moved in the peripheral and central zones, number of entries, latent period of the first entrance, and time spent in the central zone were registered (27 (link), 34 (link)).
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