Immunohistochemical Staining Evaluation

The paraffin-embedded sections were treated with immunohistochemical staining using the primary antibody EZH2 (#5246, 1:50, Rabbit, CST), HMGB1 (1:250, Rabbit, ab79823, Abcam), p-STAT3 (1:500, Rabbit, ab76315, Abcam), cleaved-caspase3 (#9661, 1:200, Rabbit, CST), and cleaved-PARP (#5625, 1:150, Rabbit, CST). The samples were observed under a Leica microscope (Leica M205C). Positive stains were scored according to a previously published method (20 (link)).

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Wang J., Li P., Xu X., Zhang B, & Zhang J. (2020). MicroRNA-200a Inhibits Inflammation and Atherosclerotic Lesion Formation by Disrupting EZH2-Mediated Methylation of STAT3. Frontiers in Immunology, 11, 907.

Publication 2020

ab79823 ab76315

Antibody Caspase3 Ezh2 Hmgb1 Microscope Paraffin Rabbit Stains Stat3

Corresponding Organization : Second Affiliated Hospital of Jilin University

Other organizations : Jilin Medical University, Jilin University

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Variable analysis

independent variables

Primary antibody EZH2 (#5246, 1:50, Rabbit, CST)
Primary antibody HMGB1 (1:250, Rabbit, ab79823, Abcam)
Primary antibody p-STAT3 (1:500, Rabbit, ab76315, Abcam)
Primary antibody cleaved-caspase3 (#9661, 1:200, Rabbit, CST)
Primary antibody cleaved-PARP (#5625, 1:150, Rabbit, CST)

dependent variables

Positive stains scored according to a previously published method (20 (link))

control variables

Paraffin-embedded sections
Leica microscope (Leica M205C)

Annotations

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