Immunostaining for Fibroblast Proliferation

Interstitial cells were labelled by immunostaining with an anti-Vimentin antibody to reveal fibroblast proliferation in the interstitium. First, the specimens were incubated with the anti-Vimentin primary antibody (1:400; Proteintech, Rosemont, IL, USA) at 4 °C overnight and each slide contained a negative control. Next, the anti-Vimentin primary antibody was visualized using a one-step polymer detection kit (Maixin, Fuzhou, China). Finally, the results were observed and evaluated as described by Liu et al. [36 (link)].

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Cheng Z., Liu L., Wang Z., Cai Y., Xu Q, & Chen P. (2018). Hypoxia Activates Src and Promotes Endocytosis Which Decreases MMP-2 Activity and Aggravates Renal Interstitial Fibrosis. International Journal of Molecular Sciences, 19(2), 581.

Publication 2018

anti-Vimentin primary antibody

Anti antibody Fibroblast Interstitial cells Polymer Vimentin

Corresponding Organization : Southeast University

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Variable analysis

independent variables

Anti-Vimentin primary antibody (1:400; Proteintech, Rosemont, IL, USA)

dependent variables

Fibroblast proliferation in the interstitium

control variables

Each slide contained a negative control

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