Antibody Generation and Reagent Procurement

The rabbit anti-NP polyclonal antibody and mouse anti-M1 monoclonal antibody were generated as previously described [71 (link)]. The rabbit anti-NS1 polyclonal antibody was generated as previously described [72 (link)]. The mouse anti-FLAG M2 monoclonal antibody (Sigma-Aldrich, USA), mouse anti-Myc monoclonal antibody (Santa Cruz Biotechnology, USA), mouse anti-His monoclonal antibody (Beyotime, China), rabbit anti-IAV PB1 polyclonal antibody (GeneTex, USA), rabbit anti-IAV PB2 polyclonal antibody (GeneTex, USA), mouse anti-Lamin B1 monoclonal antibody (Santa Cruz Biotechnology, USA), mouse anti-β-actin monoclonal antibody (TransGen Biotech, China), mouse anti-GAPDH monoclonal antibody (Abcam, UK), HRP-conjugated goat anti-mouse IgG (Beyotime, China), HRP-conjugated goat anti-rabbit IgG (Beyotime, China), HRP-conjugated rabbit anti-mouse IgG (Cell Signaling Technology, USA), and HRP-conjugated mouse anti-rabbit IgG (Cell Signaling Technology, USA) were purchased from the indicated companies. Poly(I:C) sodium salt (Sigma, USA), human IFN-β (PeproTech, USA), RNase R (Lucigen, USA), RiboLock RNase Inhibitor (Thermo Fisher Scientific, USA), protease inhibitor cocktail (Roche, Switzerland), isopropyl-β-D-thiogalactopyranoside (IPTG; Calbiochem, USA), and tolylsulfonyl phenylalanyl chloromethyl ketone (TPCK)-trypsin (Sigma-Aldrich, USA) were purchased from the indicated companies.