Adipogenic Differentiation of CD146+ Pericytes
Corresponding Organization :
Other organizations : Johns Hopkins University
Variable analysis
- CD146+ adipose pericytes from 3 to 8 passages
- Adipogenic differentiation of CD146+ adipose pericytes measured by Oil Red O staining
- Quantification of Oil Red O staining by absorbance at 548 nm
- Cell seeding density of 2 × 10^5 cells per well
- Basal medium replaced with adipogenic differentiation medium at 24 hours after seeding
- Adipogenic differentiation medium replenished every 3 days
- Oil Red O staining performed after 10 days of differentiation
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