Wild-type C57BL/6 mice and mice with floxed Atg7 (Atg7F/F) [37 (link)], with hepatic deletion of Atg7 (Atg7−/− mice) [15 (link)], with constitutively activated Nrf2 (CaNRF2) [47 (link)], or with Alb-Cre expression [48 (link)] (The Jackson Laboratory, Cat#003574) have been described previously. These mice were further crossed with mice deficient in Caspase-11(The Jackson Laboratory, Cat#024698), in Nlrp3 (The Jackson Laboratory, Cat#021302), or in Gasdermin D [14 (link)] to generate Atg7/Caspase11 −/− or Atg7/Nlrp3 −/−, or Atg7/GsdmD −/− doubly deficient mice as previously described [3 (link)]. Both male and female mice were used at the ages between 6-12 weeks. Mice were housed in a pathogen-free facility and were maintained on a 12-hour light/12-hour dark cycle with free access to food and water. The Institutional Animal Care and Use Committee (IACUC) of Tulane University and Indiana University approved all animal studies.
Free full text: Click here
