Oral Squamous Carcinoma Cell Lines

HSC-2 (oral squamous carcinoma from oral cavity), HSC-3 (oral squamous carcinoma cell line from tongue with high metastatic potential), HSC-4 (oral squamous carcinoma cell line from tongue), Ca9-22 (oral squamous carcinoma from gingiva), HO-1-u-1 (oral squamous carcinoma from mouth floor), and SAS (oral squamous carcinoma cell line from tongue) were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). CHO-K1 was obtained from the American Type Culture Collection (ATCC, Manassas, VA). CHO/hPODXL was produced in our previous study [17 (link)]. The CHO-S cell line was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). PDIS-5 (core fucose KO CHO-S) cells were established previously [48 (link)]. SAS/hPODXL-KO cells were produced using CRISPR/Cas9 plasmids (Target ID: HS0000056763) targeting human PODXL (Sigma-Aldrich Corp., St. Louis, MO, USA). HSC-2, HSC-3, HSC-4, Ca9-22, HO-1-u-1, SAS and SAS/hPODXL-KO cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM; Nacalai Tesque, Kyoto, Japan), and CHO-K1 and CHO/hPODXL were cultured in RPMI 1640 medium (Nacalai Tesque), supplemented with 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific Inc.), 100 units/mL penicillin, 100 μg/mL streptomycin, and 25 μg/mL amphotericin B (Nacalai Tesque) at 37°C in a humidified atmosphere containing 5% CO₂ and 95% air.