Protein Interaction Analysis by Co-IP and WB

Co-immunoprecipitation, immunoprecipitation and Western blot analysis were performed as described previously [5 (link)]. Antibodies reactive with human β-actin, caspase-9, caspase-3, Bcl-2, Bcl-xL, STAT1, phospho-STAT1 serine 727 (or p-STAT1 S727), phospho-STAT1 tyrosine 701 (or p-STAT1 Y701), phospho-ERK (or p-ERK), ERK, Ubiquitin (or Ub), phospho-JAK2 (or p-JAK2), JAK2, Human influenza hemagglutinin (or HA) and Poly ADP ribose polymerase (PARP) were purchased from Cell Signaling.

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Zhang Y., Chen Y., Liu Z, & Lai R. (2018). ERK is a negative feedback regulator for IFN-γ/STAT1 signaling by promoting STAT1 ubiquitination. BMC Cancer, 18, 613.

Publication 2018

human β-actin caspase-9 caspase-3 Bcl-2 Bcl-xL STAT1 p-STAT1 S727 p-STAT1 Y701 phospho-ERK Ubiquitin phospho-JAK2 p-JAK2 Poly ADP ribose polymerase (PARP)

Actin Antibodies Bcl 2 Caspase 3 Caspase 9 Co immunoprecipitation Hemagglutinin Human Human influenza Immunoprecipitation Jak2 Poly adp ribose polymerase Serine Stat1 Tyrosine Ubiquitin Western blot analysis

Corresponding Organization :

Other organizations : Shantou University Medical College, Shantou University, First Affiliated Hospital of Shantou University Medical College, University of Alberta

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Variable analysis

independent variables

Antibodies reactive with human β-actin, caspase-9, caspase-3, Bcl-2, Bcl-xL, STAT1, phospho-STAT1 serine 727 (or p-STAT1 S727), phospho-STAT1 tyrosine 701 (or p-STAT1 Y701), phospho-ERK (or p-ERK), ERK, Ubiquitin (or Ub), phospho-JAK2 (or p-JAK2), JAK2, Human influenza hemagglutinin (or HA) and Poly ADP ribose polymerase (PARP)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

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