Rabbit monoclonal antibodies for ADORA2A (ab260032) and TNFRSF18 (ab223841) were purchased from Abcam (USA). After slicing into 4-µm sections, all tissues were deparaffinized and treated with ethylenediamine tetraacetic acid (EDTA) (pH 9.0) for antigen retrieval in a microwave for 20 min. We used an Autostainer Link 48 machine (Dako, Denmark A/S, Denmark) for the staining. Subsequently, primary antibodies for ADORA2A (rabbit monoclonal, 1:100 dilution) and TNFRSF18 (rabbit monoclonal, 1:200 dilution) were added to the sections, while phosphate buffered saline (PBS) buffer was used as a blank control instead of the antibody. An EnVision Flex Kit (Dako, Denmark A/S, Denmark) was used as the second antibody. Two senior pathologists examined all cases to validate the initial scores. The percentage of positively stained cells and staining scores were used to assess the IHC results according to the methodology described in previous articles (7 (link),8 (link)). The Gene-Score = (percentage of cells of weak intensity ×1) + (percentage of cells of moderate intensity ×2) + percentage of cells of strong intensity ×3).