Cells were stained for senescence-associated β-galactosidase activity using a kit from Cell Signaling Technology (#9860) as per the manufacturer’s instructions. Ten images were acquired on a CKX41 Olympus microscope 20× objective for each experimental condition. The percentages of β-galactosidase-positive cells were scored using Image J software. Results are given as means ± S.D. of four independent experiments. The in vivo senescence assay was performed in tumor sections using SenTraGor™, a Sudan Black B analog conjugated with biotin, which reacts with lipofuscin granules that have been shown to accumulate during the senescence process (39 (link)).
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Senescence-associated β-Galactosidase Assay
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Corresponding Organization :
Other organizations : Université de Bordeaux, Inserm, Institut Européen de Chimie et Biologie, Centre National de la Recherche Scientifique, Université Paris Sciences et Lettres, Institut Curie, Université Paris Cité, Sorbonne Université, Institut de Génomique Fonctionnelle, Université Côte d'Azur, Institut Bergonié, University of Glasgow, Cancer Research UK Scotland Institute, Czech Academy of Sciences, Institute of Biophysics
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Variable analysis
independent variables
- Experimental condition
- Percentage of β-galactosidase-positive cells
- Staining protocol (using a kit from Cell Signaling Technology (#9860) as per the manufacturer's instructions)
- Microscope (CKX41 Olympus, 20× objective)
- Image analysis software (Image J)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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