Cell lines from OCCC (ES2; CRL-1978) and HG-OSC (OVCAR-3; HTB-161) were obtained from American Type Culture Collection (ATCC). Cells were maintained at 37°C in a humidified 5% CO2 atmosphere, and cultured in Dulbecco’s Modified Eagle medium (DMEM, 41965-039, Gibco, Life Technologies), containing 4.5 g/L of D -glucose and 0.58 g/L of L -glutamine, 1% FBS (S 0615, Merck), 1% antibiotic-antimycotic (P06-07300, PAN Biotech) and 0.1% gentamicin (15750-060, Gibco, Life Technologies). Cells were exposed either to 0.402 mM L -cysteine (102839, Merck) and/or to hypoxia induced with 0.1 mM cobalt chloride (CoCl2) (C8661, Sigma-Aldrich) as previously (Nunes et al., 2018a (link),b (link)).
Prior to any experiment, cells were synchronized under starvation (culture medium without FBS) for 8 h at 37°C and 5% CO2.
Prior to any experiment, cells were synchronized under starvation (culture medium without FBS) for 8 h at 37°C and 5% CO2.
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