TE analyses were performed using inductively coupled plasma mass spectrometry (ICP-MS) following Maynar et al. [17 (link)].
Briefly, to prepare the analysis, the decomposition of the organic matrix was achieved by heating it for 10 h at 90 °C after adding 0.8 mL HNO3 and 0.4 mL H2O2 to 1 mL of plasma sample. The samples were then dried at 200 °C on a hot plate. Sample reconstitution was carried out by adding 0.5 mL of nitric acid, 10 μL of indium (10 mg/L) as an internal standard and ultrapure water to bring the solution to 10 mL. Reagent blanks, element standards and certified reference materials (Seronorm, lot 0511545 Sero AS Billingstand, Norway) were prepared identically and used for accuracy testing. Before the analysis, the commercial control materials were diluted according to the manufacturer’s recommendations. Digested solutions were analyzed by an ICP-MS Nexionmodel 300D (PerkinElmer, Inc., Shelton, CT, USA). Samples were analyzed in triplicate. The values of each standard material (10 μg/L) used for quality controls were in agreement with intra and inter-assay coefficient variations of less than 5%.
Briefly, to prepare the analysis, the decomposition of the organic matrix was achieved by heating it for 10 h at 90 °C after adding 0.8 mL HNO3 and 0.4 mL H2O2 to 1 mL of plasma sample. The samples were then dried at 200 °C on a hot plate. Sample reconstitution was carried out by adding 0.5 mL of nitric acid, 10 μL of indium (10 mg/L) as an internal standard and ultrapure water to bring the solution to 10 mL. Reagent blanks, element standards and certified reference materials (Seronorm, lot 0511545 Sero AS Billingstand, Norway) were prepared identically and used for accuracy testing. Before the analysis, the commercial control materials were diluted according to the manufacturer’s recommendations. Digested solutions were analyzed by an ICP-MS Nexionmodel 300D (PerkinElmer, Inc., Shelton, CT, USA). Samples were analyzed in triplicate. The values of each standard material (10 μg/L) used for quality controls were in agreement with intra and inter-assay coefficient variations of less than 5%.
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