Probing Chondrocyte Development Genes

Probes included human CDMP1 cDNA (an ApaI fragment, residue 470– 1155) (Chang et al., 1994 (link)) and mouse α2(XI) collagen cDNA (pRAC2-28) (Tsumaki and Kimura, 1995 (link)). Mouse Ihh (Hh-14.1) and Shh (Hh-16.1) cDNA probes were provided by A. McMahon (Echelard et al., 1993 (link)). Mouse Pax1 cDNA (a HincII-SacI fragment) was obtained from H. Koseki and R. Balling (Deutsch et al., 1988 (link)). Mouse PTHrP cDNA (a AvrII/ SmaI fragment) and PTH/PTHrP receptor cDNA (a Sau3A/PvuII fragment) were obtained from A. Broadus (Mangin et al., 1990 (link)). Mouse α1(X) collagen cDNA (pRK26) was provided by K.S.E. Cheah (Kong et al., 1993 (link)). Mouse type IIA procollagen cDNA (exon 2) was from L.J. Sandell (Metsaranta et al., 1991 (link)).

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Tsumaki N., Tanaka K., Arikawa-Hirasawa E., Nakase T., Kimura T., Thomas J.T., Ochi T., Luyten F.P, & Yamada Y. (1999). Role of CDMP-1 in Skeletal Morphogenesis: Promotion of Mesenchymal Cell Recruitment and Chondrocyte Differentiation. The Journal of Cell Biology, 144(1), 161-173.

Publication 1999

Apai Cdmp1 Cdna Collagen Exon Human Mouse Procollagen Pth pthrp receptor Pthrp Smai

Corresponding Organization : Tohoku Medical and Pharmaceutical University

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Protocol cited in 7 other protocols

Variable analysis

independent variables

Human CDMP1 cDNA (an ApaI fragment, residue 470–1155)
Mouse α2(XI) collagen cDNA (pRAC2-28)
Mouse Ihh (Hh-14.1) cDNA probe
Mouse Shh (Hh-16.1) cDNA probe
Mouse Pax1 cDNA (a HincII-SacI fragment)
Mouse PTHrP cDNA (a AvrII/SmaI fragment)
Mouse PTH/PTHrP receptor cDNA (a Sau3A/PvuII fragment)
Mouse α1(X) collagen cDNA (pRK26)
Mouse type IIA procollagen cDNA (exon 2)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not mentioned

negative controls

Not mentioned

Annotations

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