Morphological and Molecular Identification of Algal Strain

Under a calibrated compound light microscope (Olympus CH20i) with 10 ×, 40 ×, and 100 × immersion lenses, the main morphological characteristics of the isolated algal strain were examined and digital photomicrographs of the specimens were taken. For molecular identification, the genomic DNA extraction method (CTAB) was used to characterize the algal strain, which was then followed by PCR, gel electrophoresis, and algal identification was done by using rbcL (Ribulose bisphosphate Carboxylase Large subunit) gene sequencing. In addition, that sequence was uploaded to the NCBI database to obtain an accession number.