We selected paraffin-embedded tissue microarray (TMA) for immunohistochemistry (IHC). IHC was performed as previously described (24 (link)). The appropriate primary antibody was added to cover the tissue, and slides were incubated at 4°C. Finally, the sections were stained with DAB Substrate Kit. The images were captured by DP Manager software (×400 magnification, OLYMPUS), and the H-score was evaluated by two independent pathologists.
Primary antibodies include the anti-PTTG1 antibody (PA5-29399, Invitrogen, USA, 1:400), anti-SLC2A1 antibody (MA5-11315, Invitrogen, USA, 1:200), anti-FAM83A antibody (SAB2108978, Sigma, USA, 1:200), anti-CD4 antibody (ab133616, Abcam, USA, 1:200), anti-CD8 antibody (SP16, Invitrogen, USA, 1:200), anti-CD20 antibody (EP459Y, Abcam, USA, 1:200), anti-CD68 antibody (KP1, Invitrogen, USA, 1:100), and anti-CD11c antibody (EP1347Y, Abcam, USA, 1:200).
Primary antibodies include the anti-PTTG1 antibody (PA5-29399, Invitrogen, USA, 1:400), anti-SLC2A1 antibody (MA5-11315, Invitrogen, USA, 1:200), anti-FAM83A antibody (SAB2108978, Sigma, USA, 1:200), anti-CD4 antibody (ab133616, Abcam, USA, 1:200), anti-CD8 antibody (SP16, Invitrogen, USA, 1:200), anti-CD20 antibody (EP459Y, Abcam, USA, 1:200), anti-CD68 antibody (KP1, Invitrogen, USA, 1:100), and anti-CD11c antibody (EP1347Y, Abcam, USA, 1:200).
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