In the morning of study Day 0, mice were placed in single chambers of a Plexiglas irradiation apparatus and exposed to a single uniform total body dose of gamma radiation from a 137Cs radiation source (GammaCell 40; Nordion International, Kanata, Ontario, Canada) at an exposure rate of 0.63-0.68 Gy/minute. Each group of mice irradiated together was roughly divided among all treatment groups to ensure that each group received the same irradiation exposure conditions. Each exposure was confirmed using Inlight Dot dosimeters (Landauer Inc.) placed inside of a parafilm mouse phantom and irradiated along with the mice. Dosimeters were read using a validated Landauer microStar reader calibrated with standard Dot dosimeters exposed with a NIST-traceable 137Cs source (Battelle Memorial Institute, WA). Reproducibility of individual dots was 3±1% with accuracy of 4±2%, well within the 10% industry standard for experimental radiation dosimetry.
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Whole-Body Gamma Irradiation of Mice
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Variable analysis
independent variables
- Gamma radiation dose from a 137Cs radiation source
- Radiation exposure conditions (radiation dose and exposure rate)
- Uniform total body dose of gamma radiation
- Exposure rate of 0.63-0.68 Gy/minute
- Irradiation of each group of mice together to ensure the same exposure conditions
- Confirmation of exposure using Inlight Dot dosimeters placed inside a parafilm mouse phantom and irradiated along with the mice
- Calibration of the Landauer microStar reader using standard Dot dosimeters exposed with a NIST-traceable 137Cs source
- Reproducibility and accuracy of the dosimeters within industry standards
- Positive control: Inlight Dot dosimeters placed inside a parafilm mouse phantom and irradiated along with the mice
- Negative control: Not mentioned
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