Statistical Analysis and Visualization

GraphPad Prism software (v9) was used when performing statistical analysis and in the creation of graphs. Where the unpaired Student’s t-test was performed for statistical analysis: **** for P<0.0001, *** for P<0.001, ** for P<0.01 and * for P<0.05 were considered statistically significant and were indicated in the related figure legends and graphs. Where n.s. is indicated, the P value is not significant. The results are shown as mean ± standard deviation (SD). CytoFlex software (Beckman Coulter) was used when performing analysis of flow cytometric data. QuPath: Open source software for digital pathology image analysis was used to perform colony quantification [23 (link)]. Empiria Studio® Software (LI-COR) was used to quantify western blot bands and normalise bands to total protein.