The “Lobato Paraense” snail facility at the René Rachou Institute—FIOCRUZ provided cercariae of S. mansoni (LE strain). The parasite cycle is maintained throughout passages between hamsters (Mesocricetus auratus) and snails (Biomphalaria glabrata).
As previously described, cercariae were mechanically transformed into schistosomula (Milligan and Jolly, 2011 (link)). Schistosomula were cultured in GMEM supplemented with 0.2 μM triiodothyronine; 0.1% glucose; 0.1% lactalbumin; 20 mM HEPES; 0.5% MEM vitamin solution; 5% Schneider’s Insect Medium; and 0.5 μM hypoxanthine, 1 μM hydrocortisone, 1% penicillin/streptomycin, and 2% heat-inactivated FBS.
Hamsters (M. auratus) were infected with cercariae and subjected to perfusion (Pellegrino and Siqueira, 1956 (link)) after 45 days for obtaining adult worms. Males and females were washed, separated manually, and cultured in Roswell Park Memorial Institute 1640 (RPMI 1640) medium supplemented with 2% penicillin/streptomycin and 10% heat-inactivated FBS.
As previously described, cercariae were mechanically transformed into schistosomula (Milligan and Jolly, 2011 (link)). Schistosomula were cultured in GMEM supplemented with 0.2 μM triiodothyronine; 0.1% glucose; 0.1% lactalbumin; 20 mM HEPES; 0.5% MEM vitamin solution; 5% Schneider’s Insect Medium; and 0.5 μM hypoxanthine, 1 μM hydrocortisone, 1% penicillin/streptomycin, and 2% heat-inactivated FBS.
Hamsters (M. auratus) were infected with cercariae and subjected to perfusion (Pellegrino and Siqueira, 1956 (link)) after 45 days for obtaining adult worms. Males and females were washed, separated manually, and cultured in Roswell Park Memorial Institute 1640 (RPMI 1640) medium supplemented with 2% penicillin/streptomycin and 10% heat-inactivated FBS.
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