Optogenetic Analysis of Synaptic Responses

The synaptic responses evoked by electrical photostimulation were measured using pClamp software (Molecular Devices). The amplitude of EPSC was measured from baseline values just before optic tract and/or photostimulation. The average of three to five trials was taken for measurements for each condition. All traces reflect the averaged responses of individual trials. To examine the changes in the synaptic response to repetitive stimulation, the percentage change in the EPSC amplitude was calculated from the initial response. To determine the degree of synaptic depression, paired-pulse ratios (PPRs) were obtained by calculating the EPSC amplitude of the 2nd or 10th response and dividing by the amplitude of the initial EPSC. Summary graphs include individual data along with mean ± SEM values. Statistical tests and levels of significance are provided in the Results. All post hoc comparisons and levels of significance indicated by asterisks are listed in the figure legends.

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Govindaiah G., Fox M.A, & Guido W. (2023). Pattern of Driver-Like Input onto Neurons of the Mouse Ventral Lateral Geniculate Nucleus. eNeuro, 10(1), ENEURO.0386-22.2022.

Publication 2023

pClamp software

Devices Electrical Optic tract Pulse

Corresponding Organization :

Other organizations : University of Louisville, Virginia Tech

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Variable analysis

independent variables

Electrical photostimulation

dependent variables

Amplitude of EPSC
Percentage change in the EPSC amplitude
Paired-pulse ratios (PPRs)

control variables

Baseline values just before optic tract and/or photostimulation
Average of three to five trials for each condition
Averaged responses of individual trials

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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