Ginsenoside Rg3 was supplied by Dr. Sung Ho Son (VitroSys Inc., Yeongju, Republic of Korea).
Zoledronate (zoledronic acid), kindly provided by Novartis Pharma AG, was added on the last day of culture to evaluate its immuno-modulatory effects on DC. The drug concentration was selected on the basis of a series of tests performed that determined the ideal concentration of Zoledronate to be 1 μmol/L.
Human Wilms’ tumor gene 1 (WT1) protein was synthesized at JW CreaGene (Seongnam, Republic of Korea). The purity of the protein was confirmed to be >95% by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). Synthetic protein was dissolved in dimethyl sulfoxide (DMSO) according to the manufacturer’s recommendations and stored at −70 °C until use.
Zoledronate (zoledronic acid), kindly provided by Novartis Pharma AG, was added on the last day of culture to evaluate its immuno-modulatory effects on DC. The drug concentration was selected on the basis of a series of tests performed that determined the ideal concentration of Zoledronate to be 1 μmol/L.
Human Wilms’ tumor gene 1 (WT1) protein was synthesized at JW CreaGene (Seongnam, Republic of Korea). The purity of the protein was confirmed to be >95% by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). Synthetic protein was dissolved in dimethyl sulfoxide (DMSO) according to the manufacturer’s recommendations and stored at −70 °C until use.
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