The myeloperoxidase–DNA (MPO–DNA) complexes were quantified using the Quant-iT PicoGreen double stranded DNA (dsDNA) assay kit (Invitrogen, USA) according to the manufacturer's instructions [17 (link)]. To quantify NETs in the cell culture supernatant and plasma, a capture ELISA of myeloperoxidase (MPO) associated with DNA was performed. For the capture antibody, an MPO kit (Hycult Biotech, HK210-01) was used.
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