Western Blot Protein Detection

Western blot analyses were performed using rabbit anti-RhsA (diluted 1:5,000, Genscript, custom polyclonal [14 (link)]), rabbit anti-FLAG (diluted 1:5,000, Sigma), rabbit anti-VSV-G (diluted 1:5,000; Sigma) and mouse anti-His₆ (diluted 1:5,000, Genscript) and detected with anti-rabbit or anti-mouse horseradish peroxidase-conjugated secondary antibodies (diluted 1:5,000; Sigma). Development of western blots was completed using chemiluminescent substrate (Clarity Max, Bio-Rad) and imaged with the ChemiDoc Imaging System (Bio-Rad).

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Günther P., Quentin D., Ahmad S., Sachar K., Gatsogiannis C., Whitney J.C, & Raunser S. (2022). Structure of a bacterial Rhs effector exported by the type VI secretion system. PLoS Pathogens, 18(1), e1010182.

Publication 2022

rabbit anti-FLAG rabbit anti-VSV-G anti-rabbit or anti-mouse horseradish peroxidase-conjugated secondary antibodies Clarity Max ChemiDoc Imaging System

Anti antibodies Horseradish peroxidase Mouse Rabbit Western blots

Corresponding Organization : Discovery Centre

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Variable analysis

independent variables

Rabbit anti-RhsA (diluted 1:5,000, Genscript, custom polyclonal [14 (link)]))
Rabbit anti-FLAG (diluted 1:5,000, Sigma)
Rabbit anti-VSV-G (diluted 1:5,000; Sigma)
Mouse anti-His6 (diluted 1:5,000, Genscript)

dependent variables

Western blot analyses

control variables

Anti-rabbit or anti-mouse horseradish peroxidase-conjugated secondary antibodies (diluted 1:5,000; Sigma)
Chemiluminescent substrate (Clarity Max, Bio-Rad)
ChemiDoc Imaging System (Bio-Rad)

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