Total RNA from chordoma cells (U-CH1 or MUG-Chor1) and tissues frozen in liquid nitrogen was isolated using the RNA extraction kit (Qiagen, Germantown, MD, USA, Cat# 74134) and TRIzol reagent. The DNA micro kit (Qiagen, Cat# 56304) was applied to isolate gDNA. The concentration of the extracted RNA was measured using NanoPhotometer Pearl (IMPLEN, Schatzbogen, Munchen, Germany, Model# 03384).
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