Intravenous Nicotine Administration and Neurotransmitter Antagonists

The (−)-Nicotine hydrogen tartrate salt (Sigma-Aldrich) was dissolved in sterile physiological saline (0.9% NaCl) and administered intravenously at a concentration of 30.0 μg/kg/0.1 ml infusion. The pH of the solution was adjusted to 7.4 with NaOH 5 m. The GABAA receptor antagonist bicuculline-methiodide (bicuculline) was diluted in Milli-Q to 20 mm and further diluted in artificial CSF (aCSF; 20 μm), while the AMPA receptor antagonist CNQX was dissolved in aCSF (10 μm) shortly before use. All drugs were purchased from Sigma-Aldrich.

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Domi A., Domi E., Lagstrom O., Gobbo F., Jerlhag E, & Adermark L. (2023). Abstinence-Induced Nicotine Seeking Relays on a Persistent Hypoglutamatergic State within the Amygdalo-Striatal Neurocircuitry. eNeuro, 10(2), ENEURO.0468-22.2023.

Publication 2023

(−)-Nicotine hydrogen tartrate salt

0 9 nacl Ampa receptor Bicuculline Bicuculline methiodide Cnqx Drugs Gabaa receptor antagonist Hydrogen Nicotine tartrate Physiological Saline Salt Sterile

Corresponding Organization :

Other organizations : University of Gothenburg, Università di Camerino, University of Edinburgh

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Variable analysis

independent variables

(−)-Nicotine hydrogen tartrate salt concentration (30.0 μg/kg/0.1 ml infusion)
GABAA receptor antagonist bicuculline-methiodide (bicuculline) concentration (20 mM in Milli-Q, further diluted in artificial CSF to 20 μM)
AMPA receptor antagonist CNQX concentration (10 μM in artificial CSF)

dependent variables

Not explicitly mentioned

control variables

PH of the (−)-Nicotine hydrogen tartrate salt solution (adjusted to 7.4 with NaOH)
Sterile physiological saline (0.9% NaCl) used as the vehicle for (−)-Nicotine hydrogen tartrate salt
Artificial CSF (aCSF) used as the vehicle for bicuculline and CNQX

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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