Golgi-Type II Neuron Staining

A block (4 mm thick) containing the AV thalamic nucleus was cut, dried, and kept in a mixture of 3% zinc chromate (CrO₄Zn; 11483697, Thermo Fisher Scientific Inc., Dreieich, Germany) and 2% formic acid (CH₂O₂; 15508664, Thermo Fisher Scientific Inc., Germany) for 7 days. Then, the block was removed from the chromate solution, dried without washing, and immersed in a 0.75% silver nitrate solution (AgNO₃; 4500.1, Carl Roth GmbH, Germany) for another 7 days. This method gave the best results regarding the impregnation of axons of Golgi-type II neurons, with impregnation of very few neurons with a very clear background.

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El-Dwairi Q.A., Al-Hussain S.M., Banihani A.S., Bataineh Z.M., Djouhri L., Mustafa A.G, & Zaqout S. (2023). Neuronal Cell Types in the Spinal Trigeminal Nucleus of the Camel Brain. Brain Sciences, 13(2), 312.

Publication 2023

AgNO3

Axons Chromate Formic acid Golgi Impregnation Neurons Silver nitrate Thalamic nucleus Zinc chromate

Corresponding Organization : Qatar University

Other organizations : Jordan University of Science and Technology

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Variable analysis

independent variables

Immersion time in 3% zinc chromate (CrO4Zn) and 2% formic acid (CH2O2) solution
Immersion time in 0.75% silver nitrate (AgNO3) solution

dependent variables

Quality of impregnation of axons of Golgi-type II neurons
Number of neurons impregnated

control variables

Thickness of the block (4 mm)
Composition of the chromate and formic acid solution (3% zinc chromate and 2% formic acid)
Concentration of the silver nitrate solution (0.75%)

controls

Positive control: The method gave the best results regarding the impregnation of axons of Golgi-type II neurons, with impregnation of very few neurons and a very clear background.

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