MTT Cell Viability Assay for HT22 Cells

Cell viability was determined using the MTT Cell Viability Assay kit (Beyotime Institute of Biotechnology) according to the manufacturer's instructions (16 (link)). HT22 cells were seeded at a density of 3×10³ cells/well into 96-well-plates and cultured with different concentrations of DMY (0, 10, 30, 100 or 300 µmol/l) for 24 h at 37°C. Cells were exposed to OGD/R prior to a 4-h incubation with 20 µl MTT solution administered to each well. DMSO was then added to each well to dissolve the formazan particles. The absorbance in each sample was then measured at a wavelength of 450 nm using a microplate reader (Thermo Fisher Scientific, Inc.). Each assay was performed in triplicate.