Cytokine Production Assay Protocol

Peripheral blood cells or splenocytes were restimulated with indicated peptides in the presence of Golgi-Stop (Biolegend) for 4 hours and intracellularly stained for interferon-gamma (IFNγ) or IL-2 as described.^{(34 (link))} Enzyme-linked immunosorbent assay (ELISA) of IFNγ and IL-2 was conducted per the instructions (Biolegend).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Zhu W., Peng Y., Wang L., Hong Y., Jiang X., Li Q., Liu H., Huang L., Wu J., Celis E., Merchen T., Kruse E, & He Y. (2018). Identification of α-Fetoprotein-Specific T-Cell Receptors for Hepatocellular Carcinoma Immunotherapy. Hepatology (Baltimore, Md.), 68(2), 574-589.

Publication 2018

Golgi-Stop

Enzyme linked immunosorbent assay Golgi Ifnγ Peptides Peripheral blood cells

Corresponding Organization : Augusta University

Other organizations : Peking University First Hospital, Peking University, Newcastle University

Top 5 similar protocols

Variable analysis

independent variables

Indicated peptides

dependent variables

Interferon-gamma (IFNγ)

control variables

Presence of Golgi-Stop (Biolegend)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!