Fluorescent In Situ Hybridization Analysis of Met, GFP, and Chat in Met-EGFP Mouse Brains

Fresh frozen brains from Met^EGFP mice were cryosectioned at 16μm and processed according to a commercially available RNAscope Multiplex Fluorescent Assay Kit (Advanced Cell Diagnostics, Hayward, CA) as described previously (Kast, Wu, Williams, Gaspar, & Levitt, 2017 (link)). RNAscope probes used in this study include: Met (cat# 405301), Gfp (cat# 400281), and Chat (cat# 410071).

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Kamitakahara A., Wu H.H, & Levitt P. (2017). Distinct Projection Targets Define Subpopulations of Mouse Brainstem Vagal Neurons that Express the Autism-Associated MET Receptor Tyrosine Kinase. The Journal of comparative neurology, 525(18), 3787-3808.

Publication 2017

RNAscope Multiplex Fluorescent Assay Kit

Assay Brains Cell Diagnostics Frozen Mice

Corresponding Organization : University of Southern California

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Variable analysis

dependent variables

Expression of Met, Gfp, and Chat genes

control variables

Fresh frozen brains from Met^EGFP mice
Cryosectioning at 16μm
RNAscope Multiplex Fluorescent Assay Kit

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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